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O’Brien Core Center for Acute Kidney Injury Research
(NIH Grant number:P30 DK079337)
REQUEST FOR CORE USAGE FORM

Please complete the online form below, or print the pdf version and submit to:
Ms. Audrey Moore Burnett
Email: aamoore@uab.edu
Fax: (205) 996 6650

 

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NIH      Other

Check desired core resources for your project and any other pertinent information.

Biostatistical/Bioinformatics Resource (BBR)

Contact: Gary Cutter, Ph.D. (cutterg@uab.edu)
  • Analysis of pilot data
  • Design of Epidemiological, Clinical studies or Clinical trials
  • Sample Size and/or Power for trials
  • Analyses of study results
  • Database development
  • Forms and/or instrument development

Core A: Resource for Clinical studies of Acute Kidney Injury

Contact: Ravindra Mehta, M.D. (rmehta@ucsd.edu)
  • Study Planning and Design
    • Protocol creation, IND submission, IRB/GCRC submission, Budget creation, Patient recruitment plans, Informed consent form creation, Study registration
    • Ethics Issues: Ethical considerations for study design, Optimization of informed consent documents and process, Safety - Research Safety Advisor related issues and procedures (e.g. establishment of a Data Safety Management Panel)
  • Study Initiation and Conduct
    • Data Base Management System: Define variables, Creation of CRF’s, web based relational database, Manuals for Standard operating procedures
    • Training for using database, study monitoring procedures, study conduct
    • Tools for monitoring study conduct and quality assessment with GCP guidelines
    • Ongoing safety data accrual and reporting
    • Data compilation and preparation for data analysis
  • Study Analysis and Interpretation (In conjunction with BBR)
    • Primary study data analysis and interpretation, analysis of data from translational Studies, plans for follow up studies.
  • Biomarkers for AKI
    • Functional markers: Serum creatinine (HPLC), Cystatin C, beta-2 microglobulin
    • Structural markers: NGAL, KIM-1, IL-18, Osteopontin, MMP, albumin, TGF-β
    • Cytokines: TNF α, IL-6, IL-10, IL-1 and additional assays available on Luminex platform
    • Assays available on Meso Scale Diagnostics Platform
  • Biological Sample Repository
    • Procedures for sample acquisition
    • Barcoding system for lab samples integrated with database
    • Kits for sample acquisition and shipping
    • Sample storage
  • Design of Genetic studies of AKI
    • Design of single nucleotide polymorphism assays
    • Preparation of genomic DNA, with quality control.
    • Performance of single nucleotide polymorphism assays.
    • QC of SNP assays.
    • Informatics and statistical genetics of SNP information.

Core B: Resource for Pre-Clinical studies of Acute Kidney Injury

Contact: Paul W. Sanders, M.D. (psanders@uab.edu)
  • Rodent (mouse and rat) models of transplantation
    • Orthotopic kidney transplantation
    • Heterotopic heart transplantation
    • Orthotopic abdominal aorta transplantation
  • Rodent models of acute kidney injury
    • Renal ischemia/reperfusion
    • Nephrotoxin-induced models of AKI (e.g. cisplatin, gentamicin)
    • Rhabdomyolysis model of AKI
  • Other procedures (e.g. tail vein injection, cannulation)
  • Arterio-venous fistula (carotid artery to jugular vein)
  • Use of microsurgical workstation
  • Hands-on training of a surgical protocol
  • Isolation and culture of primary cell lines (proximal tubule, endothelial, smooth muscle)
  • Functional renal imaging analysis
  • Structural and metabolic imaging
    • Ultrasound
    • MicroCT
  • Gamma-ray Imaging
    • Gamma camera
    • SPECT/CT
  • Optical Imaging
    • Bioluminescence
    • Fluorescence
  • Renal Physiological Techniques in rodents
    • Whole kidney clearance and oxygen consumption studies
    • Tissue oxygen partial pressure determination in kidney
    • Nitric oxide generation
    • In vitro assessment of O2 consumption by isolated proximal tubule segments
    • Micropuncture for nephron function and tubuloglomerular feedback
    • Metabolic cage experiments in mice
  • Radioimmunoassay of angiotensin II
  • Videometric flow velocitometry (VMFV)

Core C: Bioanalytical Resource

Contact: Stephen Barnes, Ph.D. (sbarnes@uab.edu)
  • Training in proteomics and mass spectrometry approaches
  • Consultation for analysis of oxidative stress biomarkers in AKI
  • Serum/plasma creatinine determination by isotope dilution LC-MS/MS
  • Assays for oxidative stress markers
    • F2-isoprostanes
    • Sulfhydryl and oxidative modification of proteins and peptides
  • Bioenergetic analysis of cultured cells
  • Assays for post-translational modification of proteins
    • 2D-gel approaches (e.g. CyDye, 2D, DIGE, blue native 2D gels)
    • Protein identification by peptide mass fingerprint analysis (MALDI-TOF-TOF MS)
    • Confirmation of protein modifications and quantitative analysis (LC-MS/MS)
  • Molecular pathology (e.g. laser capture microdissection, in situ hybridization)
Please acknowledge the UAB-UCSD O’Brien Core Center (NIH 1P30 DK 079337) in any publications that result from the use of the core services.