Bioanalytical Resource (proteomics/oxidative stress markers/ molecular pathology)
Director:
Stephen Barnes, Ph.D., Univ of Alabama at Birmingham
Co-Directors:
Victor Darley-Usmar, Ph.D., Univ of Alabama at Birmingham
Gene Siegal, M.D., Ph.D., Univ of Alabama at Birmingham
Daniel Sharer, Ph.D., Univ of Alabama at Birmingham
The objective of the Bioanalytical Core (Core C) is to provide state-of-the-art mass spectrometry and proteomics support for investigators carrying out AKI research at UAB and UCSD as well as investigators of the Extended Research Base. An important part of this support is to provide a comprehensive resource that will allow access to optimized protocols and technology for the detection and characterization of changes occurring in AKI and advanced resources/protocols for bioanalytical analyses using proteomics and mass spectrometry approaches. This aspect will be integrated into a comprehensive educational activity that promotes existing core services, and updates on evolving proteomics technology. Because analytical technologies are continually evolving, an important role of the Core will be to keep AKI investigators appraised of technological developments in the field through a program of tutorials and seminars; this will be especially important for AKI investigators who have not previously used technologies offered by the core. Information will be disseminated from ongoing and completed experiments in Core C through posting to an existing proteomics database (http://www.uab.edu/proteomics) established through the UAB Proteomics/Mass Spectrometry Shared Facility. Using this approach AKI investigators both within and outside UAB can not only monitor progress of their sample analysis, but also view proteomics data from other experiments analyzed by the Core. The goal of the Core is to provide a molecular underpinning for research on AKI performed in this Center.